An Introduction to Microbiology. Pharmaceutical Monographs by W. B. Hugo, J. B. Stenlake

By W. B. Hugo, J. B. Stenlake

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Attempts to explain why in one group of bacteria, after treatment with a dye and iodine, the colour may be washed from the cell with ethanol and in another group it cannot, may be divided into two categories. In the first category it is suggested that there is a special cellular component which after treatment with the dye and iodine so effectively combines with the dye that its subsequent solution in the decolorising solvent does not occur. Cellular components that have been suggested are lipids, nucleic acids, carbohydrates, iipo- and nucleo-proteins and glycerophosphates.

E. and F. Spon, 1968 The Bacterial Spore. Ed. Gould, G. W. and Hurst, A. Academic Press, 1969 36 CHAPTER 2 BACTERIAL GROWTH AND FACTORS AFFECTING BACTERIAL GROWTH THE CULTIVATION OF BACTERIA The bacterial cell has so far been considered as a single entity; normally, cells are seen collectively as aggregations of many cells on a solid medium which are known as bacterial colonies or in liquid culture conferring turbidity on an originally optically clear medium. The morphology of bacterial colonies is of importance in systematic bacteriology.

Aureus + — — — + + * + + + ? + N. meningitidis Pr. vulgaris 1 1 Sal. typhosa I 1 Pr. morganii Es eh. coli 1 Glycine Alanine Serine Cystine Phenylalanine Tyrosine Tryptophan Threonine Valine Leucine Isoleucine Glutamic acid Aspartic acid Histidine Lysine Arginine Methionine Proline Hydroxyproline Glutamine Asparagine 1 BACTERTA — — — — — * 7 — — + — * + —+ — + + — — * + —+ — + + — — + — — — — — — ? — + — — — + —+ — — + — — — — — — + +7 ? 7 7 + + ——+ + + + + + _ 7 + + + +') + 7 _ — 9 — — 7 — + + — 7 — + + + — ?

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