By M.V. Nermut and A.C. Steven (Eds.)
Animal Virus constitution presents a entire, state of the art precis of the constitution, molecular composition, and crucial antigenic and organic homes of every at present famous kinfolk of animal viruses. info deriving from electron microscopy, laptop photograph processing and X-ray diffraction is built-in systematically with biochemical information into three-d molecular versions of viral structure. a quick account of virus/cell interplay and pathogenicity completes every one bankruptcy.
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Additional info for Animal Virus Structure
1983) Study of negatively stained images of Sendai virus nucleocapsids using minimum-dose system. J . Ultrastruct. Res. 84, 140-150. P. B. , AppletonCentury-Crofts, Norwalk, CT. Kaper, J. (1975) The chemical basis of virus structure, dissociation and reassembly. North Holland, Amsterdam. Liljas, L. (1986) The structure of spherical viruses. Prog. Biophys. Mol. Biol. 48, 1-36. Klug, A. (1983) Architectural design of spherical viruses. Nature 303, 378-379. , Horne, R. and Tournier, P. (1962) A system of viruses.
Courtesy of Dr. H. Frank. 26 with uranyl acetate. Stain-penetrated particles are much smaller than those which are only stained on the surface and therefore displaying their surface projections. Surface properties of the supporting films can infuence the final image. The meniscus of the negative stain around the virus particle or macromolecule depends on the surface properties of both the protein structure and the supporting film (hydrophilic or hydrophobic; see diagram in Nermut 1982a). Hydrophobic patches can develop within several days on glow-discharged films.
Frank. g. nucleic acids) or for periodic structures such as 2D regular arrays of virus particles. The improved resolution of heavy metal shadowing (2 nm or less) has been achieved thanks to the introduction of electron beam evaporators allowing the use of metals with very high melting points such as Pt, W or Ta/W. Fig. 15 in the chapter on Iridoviridae shows the surface structure of Frog virus 3 after freeze-etching and shadowing with Pt/C. 5 nm. Very good results have also been obtained with adenoviruses (Fig.